Wet amounts and Hanging Drops Lab Report Example | Topics and Well Written Essays - 500 words

Wet amounts and Hanging Drops - Lab Report Example On the other hand, wet amount method comprises of a very tiny film of liquid sandwiched between a microscopic and a cover slip respectively (Pommerville& Alcamo, 2010). In the two cases, temperature generated from the lamp and concentrated on the slide by the condenser will start to destroy the bacteria within the shortest time possible. Notably, the light should not be on if there is nobody observing the slides. If the cells are not available shortly; a new slide should be made in order to measure motility precisely. Preparation of hanging drop: to perform this experiment, a small amount of Vaseline was placed at both ends of the cover slip using a small stick. A tiny wax pencil spot was also placed close to one corner to assist in focusing. A little drip of culture was then placed in the middle of the cover slip. For the Vaseline to be in touch with the slide and the depression to be above the culture drop, the depression slide was upturned over the cover slip (Pommerville& Alcamo, 2010). The slide was taken and overturned slowly and cautiously to ensure that the position of the culture drop does not change. This was meant to assist in making observations close to the edge of the culture drop and not in the middle. Preparation of wet amount: the preparation involved the creation of a single tiny drip of culture from numerous loopfuls. This was then followed by gripping a cover slide perpendicularly to the main slide while holding the opposite phase of the cover slide that does not have a drop. After that, the coverslip was gradually and cautiously joined with the main slide to ensure that the drip is equally distributed between the slide and coverslip (Pommerville& Alcamo, 2010). Notably, to prevent the coverslip from floating and complicating the process of focusing, only a small amount of liquid was required. Since it is not easy to observe unstained bacteria, using little water was